Mechanism of Gualoupi Injection in treatment of atherosclerosis based on network pharmacology and animal experiments
Objective To investigate the mechanism of action of Gualoupi Injection in treatment of atherosclerosis based on network pharmacology and animal experiments.Methods To search the chemical composition reports of Gualoupi Injection,and predict the active constituents and targets by Swiss ADME and Swiss Target Prediction.The targets of atherosclerosis were obtained by OMIM,TTD,and GeneCards databases,and the common targets of Gualoupi Injection for the treatment of atherosclerosis were obtained by Venny 2.1 software.Cytoscape 3.6.1 software was used to construct the"drug-component-target"network,String and DAVID databases were used to construct the PPI network,GO and KEGG enrichment analysis.The atherosclerosis mouse model was established by high-fat diet,and the mice were randomly divided into control group,model group,Gualoupi Injection(0.6,1.2 mL/kg)group,with 6 mice in each group.Gualoupi Injection group was given 0.6 and 1.2 mL/kg of Gualoupi Injection,respectively,for 3 weeks.The relevant indexes of blood lipids were detected by automatic biochemical analyzer,the pathological changes of aorta were observed by HE staining,and the expression of core proteins was detected by Western blotting.Results 21 Active components of Gualoupi Injection,including apigenin-7-O-β-D-glucoside,luteolin,and vanillic acid were selected,with 89 targets.The core targets included Akt1,Caspase 3,and SRC.GO functional analysis obtained 702 items,KEGG enrichment obtained 42 signaling pathways.Animal experiments showed that the levels of TC,TG,and LDL-C in serum could be significantly reduced by Gualoupi Injection(P<0.05,0.01).HE staining showed that Gualoupi Injection could reduce plaque adhesion on the active vascular wall.Western blotting showed that Gualoupi Injection significantly increased the protein expression of Caspase 3,and decreased the protein expressions of p-SRC and p-Akt1(P<0.05,0.01).Conclusion Gualoupi Injection regulates Caspase 3,p-SRC,p-Akt1 and other targets through multiple components,thereby reducing blood lipids and plaque attachment,and thus treating atherosclerosis.
NETL
NSTL
万方数据
维普
