Study on the mechanism of Zhibai Dihuang Pills in treatment of endocrine resistance in breast cancer based on network pharmacology
Objective To explore the active ingredients,potential targets and mechanism of action of Zhibai Dihuang Pills in treatment of endocrine drug resistance in breast cancer by network pharmacology.Methods Through TCMSP database,TCM-ID database and BATMAN-TCM database,the active ingredients and targets of Zhibai Dihuang Pills was screened.And the targets related to drug resistance of breast cancer endocrine therapy were retrieved by GeneCards database,OMIM database,TTD database and GEO database.The intersection of component targets and disease targets was used to obtain common targets.The protein-protein interaction network of Zhibai Dihuang Pills for the treatment of endocrine resistance of breast cancer was constructed by String 11.5 database,and the core targets were screened by CytoNCA plug-in of Cytoscape 3.8.2 software.The GO and KEGG pathway enrichment analysis of intersection targets was performed by Metascape gene function annotation analysis tool.The interaction network of active ingredient-target-pathway was constructed by Cytoscape 3.8.2 software to obtain the core active ingredients.Results A total of 80 active components of Zhibai Dihuang Pills were screened,and 117 intersecting targets with diseases were screened,mainly involving TP53,Akt1,TNF,IL-6,ESR1,MAPK1 and so on.KEGG pathway enrichment analysis showed that PI3K/Akt,MAPK,NF-κB and estrogen may be the key signal pathways of Zhibai Dihuang Pills in the treatment of breast cancer endocrine resistance.Quercetin,kaempferol,dehydrated icariin,β-sitosterol and diosgenin are the core active components from the drug active component-target-pathway network.Conclusion Core active ingredients such as quercetin,kaempferol,Anhydroicaritin,β-sitosterol and diosgenin in Zhibai Dihuang Pills may affect the signaling pathways of PI3K/Akt,MAPK,NF-κB and estrogen by acting on multiple targets such as TP53,Akt1,TNF,IL-6,ESR1 and MAPK1,which plays a role in the treatment of breast cancer endocrine resistance.
NETL
NSTL
万方数据
维普
