Evaluation of Rubiae Radix from different regions by HPLC multi index components combined with chemometrics
Objective To establish a method for quality evaluation of Rubiae Radix by HPLC combined with chemometrics.Methods The contents of neohesperidin,kaempferol,alizarin,xanthopurpurin,purpurin,1,3,6-trihydroxy-2-methylanthraquinone,mollugin,dehydro-α-lapachone,β-sitosterol,and isolariciresinol in 18 batches of Rubiae Radix collected from 8 provinces were determined by HPLC method,and the results were analyzed by principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and grey relational analysis(GRA)method.Results Rubiae Radix from the same production area had similar quality.A total of four differential marker components were found,and their significance was ranked as follows:mollugin>alizarin>1,3,6-trihydroxy-2-methylanthraquinone>purpurin.The overall quality of Rubiae Radix in Shaanxi,Henan,and Anhui Province was good.Conclusion The method is convenient,accurate,and easy to promote,which can comprehensively and systematically evaluate the quality of Rubiae Radix.
NETL
NSTL
万方数据
维普
