Effect and mechanism of Bufei Huoxue Capsules on pulmonary fibrosis in mice
Objective To investigate the effect and mechanism of Bufei Huoxue Capsules on pulmonary fibrosis in mice.Methods A total of 90 C57BL/6J mice was randomly divided into control group,model group,NLRP3 inhibitor(MCC950,10 mg/kg),and Bufei Huoxue Capsules(0.32,0.63,and 1.62 g/kg)group,each group had 15 mice.Mice were given a single intratracheal injection of 2.5 mg/kg bleomycin to induce pulmonary fibrosis,mice in the control group received a single intratracheal injection of an equal volume of PBS.The drug was administered first day after successful modeling,and the whole administration process lasted for 21 d.The survival rate and body weight of mice were counted.Bronchoalveolar lavage fluid(BALF)was collected and the total cell count and total protein were determined.The wet/dry weight ratio(W/D)and hydroxyproline(HYP)of lung tissue were measured.The pathological changes of lung tissue were observed by hematoxylin and eosin(HE)staining and Masson trichrome staining.qRT-PCR was used to detect the mRNA expression of EMT-related proteins(E-cad,VIM,α-SMA,and TGF-β1).Western blotting was used to detect the expression of EMT-related proteins and NLRP3 pathway-related proteins(NLRP3,ASC,mature-Caspase-1,mature-IL-1β,mature-IL-18).Results Compared with the model group,the survival rate of pulmonary fibrosis mice after MCC950 and Bufei Huoxue Capsules intervention was significantly improved,the body weight was significantly increased,the W/D of lung tissue,the total cell count and total protein and HYP content of BALF were significantly decreased(P<0.05,0.01),the pathological morphology of lung tissue was significantly improved,the expression of epithelial cell marker E-cad mRNA and protein in lung tissue was increased,the expression of mesenchymal cell marker Vim,α-SMA,TGF-β1 mRNA and protein was decreased,and the expression of related proteins in NLRP3 signaling pathway was decreased(P<0.05,0.01).Conclusion Bufei Huoxue Capsules can improve bleomycin-induced pulmonary fibrosis in mice by inhibiting NLRP3 pathway-mediated EMT.
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