Bioinformatics and experimental verification to explore the anti-cervical cancer effect and mechanism of oroxylin A
Objective To investigate the inhibitory effect and potential mechanism of oroxylin A on cervical cancer.Methods The expression difference of SHCBP1 between cervical cancer patients and healthy people was analyzed in GEO and TCGA datasets.The inhibitory effect of oroxylin A on Hela cells was verified by MTT assay.Western blotting experiments were used to study the relationship between oroxylin A and SHCBP1,and molecular simulation docking was used to evaluate the docking effect between oroxylin A and SHCBP1.The co-expression gene of SHCBP1 in patients with cervical cancer was analyzed by Linkedomics.Gene Ontology(GO)and Kyoto Genome Encyclopedia(KEGG)were used to analyze the underlying mechanisms of related co-expressed genes.CIBERSORT method was used to analyze the correlation between SHCBP1 and immune cell infiltration of cervical cancer.TISIDB database was used to analyze the relationship between SHCBP1 and immune checkpoint.Results The analysis results of GEO database and TCGA database showed that SHCBP1 was highly expressed in cervical cancer patients.The results of MTT and Western blotting analysis showed that oroxylin A had significant inhibitory effect on the expression of Hela cells and SHCBP1 at the concentration of 10 μmol/L(P<0.05).The results of molecular docking showed that the combination of oroxylin A and SHCBP1 had good potential energy.The results of TISIDB database and CIBERSORT analysis of GEO data show that SHCBP1 is involved in the immune invasion of cervical cancer cells.SHCBP1 and its co-expression genes are mainly enriched in inflammatory pathways.Conclusion Oroxylin A suppresses the growth of cervical cancer by suppressing SHCBP1-mediated immune infiltration,which could potentially have significant implications in the treatment of cervical cancer.
NETL
NSTL
万方数据
