Mechanism of Xihuang Pills in treatment of cervical cancer based on network pharmacology and molecular docking technology
Objective To explore the mechanism of Xihuang Pills in treatment of cervical cancer through pharmacology network and molecular docking methods.Methods To search the active ingredients and targets of Xihuang Pills by TCMSP and BATMAN-TCM databases.To obtain the disease targets of cervical cancer OMIM and GeneCards databases.After the combination and deduplication,it intersected with the effective ingredient target obtained by Xihuang Pills.The protein interaction(PPI)network map was constructed using String database.Cytoscape 3.9.1 software was used for visualization,and the intersection genes were analyzed using DAVID database for functional enrichment(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.Finally,AutoDock Vina and Pymol software were used to verify the molecular docking.Results Seventy main active components of Xihuang Pills were screened,including 3beta-hydroxy-5alpha-androstan-17-one,quercetin,androsterone,etc.There are 116 targets associated with cervical cancer,the core targets are TP53,EGFR,Akt1,IL-6,MYC,JUN,CASP3,IL-1B,TNF,ESR1,Bcl-2,HIF-1A,MMP9,RELA,etc.GO and KEGG analysis results showed that the important pathways of Xihuang Pills in treatment of cervical cancer were IL-17,TNF,PI3K/Akt,p53,HIF-1,and other signaling pathways.Conclusion Xihuang Pills mainly treats cervical cancer by regulating IL17,TNF,PI3K/Akt,p53,HIF-1 signaling pathways.
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