抑制电导离子色谱法测定醋酸钙颗粒中钙离子
Determination of calcium ion in Calcium Acetate Granules by ion chromatography-suppressed conductivity method
葛薇薇 1吴珺 1朱静1
作者信息
- 1. 泰州市药品检验院,江苏 泰州 235500
- 折叠
摘要
目的 建立采用抑制电导离子色谱法测定醋酸钙颗粒中钙离子的方法.方法 采用Dionex IonPacTM CS12A色谱柱(250 mm×4 mm),Dionex IonPacTM CG12A保护柱(50 mm×4 mm),以 0.02 mol/L甲烷磺酸溶液为洗脱液,体积流量 1.0 mL/min,柱温 30℃,检测池温度 35℃,电导检测器,抑制器为Dionex CDRS 600 4 mm,电流值 59 mA,进样体积为 25 μL.结果 钙在 0.002～6.000 µg/mL线性关系良好(r=0.999 7),平均回收率为 104.6%,RSD值为 1.8%.结论 方法简单、专属性高、准确可靠,能够有效地控制醋酸钙颗粒中钙离子.
Abstract
Objective To establish a method of ion chromatography-suppressed conductivity to determine calcium ion in Calcium Acetate Granules.Methods The assay was performed on Dionex IonPacTM CS12A column(250 mm×4 mm)with Dionex IonPacTM CG12A column(50 mm×4 mm)as guard column.Mobile phase was used as 0.02 mol/L methanesulfonic acid solution.Flow rate was 1.0 mL/min,column temperature was 30℃,and detecting pool temperature was 35℃.Conductivity detector was used with CDRS 600 4 mm as suppressor,current value was 59 mA,and injection volume was 25 μL.Results Calcium showed good linear relationships of 0.002—6.000 μg/mL(r=0.999 7),whose average recovery was 104.6% with RSD values of 1.8%.Conclusion The method is simple,highly specific,accurate and reliable,and can effectively control calcium ion in Calcium Acetate Granules.
关键词
醋酸钙颗粒/钙/抑制电导离子色谱法Key words
Calcium Acetate Granules/calcium/ion chromatography-suppressed conductivity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据