Study on Compound Lishao Tablets in treatment of primary trigeminal neuralgia in rats
Objective To investigate the therapeutic effect of Compound Lishao Tablets on primary trigeminal neuralgia in rats and explore the related mechanism.Methods Primary trigeminal neuralgia model established by chronic constriction injury of the infraorbital nerve,and then these rats were randomly divided into model group,carbamazepine group,low-dose Compound Lishao Tablets group,middle-dose Compound Lishao Tablets group,high-dose Compound Lishao Tablets group for 28 d gavage administration.The mechanical pain threshold(MWT)of the operated side was measured regularly,the levels of TNF-α and IL-1β in serum were detected by ELISA kit,the expression of TNF-α and IL-1β mRNA in trigeminal ganglion was detected by q-PCR,the expression of calcitonin gene related peptide(CGRP)in trigeminal ganglion was detected by Western blotting,and the expression of phosphorylated p38 mitogen activated protein kinase(p-p38 MAPK)in cerebral striatum was detected by immunohistochemistry.Results On the day after operation,MWT at the operative side of rats in all groups was significantly decreased compared with that before operation(P＜0.05),primary trigeminal neuralgia modeling is successful.The MWT value of Compound Lishao Tablet high-dose group was 19.58,which was increased by 36.6%compared with model group(P＜0.05),and Compound Lishao Tablets significantly improved the MWT value of primary trigeminal neuralgia model rats.Compared with the carbamazepine group(14.17),the MWT of rats in the middle-dose Compound Lishao Tablets group and high-dose Compound Lishao Tablets group significantly increased.Compared to the model group,the expression of TNF-α,IL-1β in serum,the level of TNF-α,IL-1β mRNA,CGRP in trigeminal ganglion and the p-p38 MAPK in striatum are significantly decreasing(P＜0.05)of middle-dose Compound Lishao Tablets group and high-dose Compound Lishao Tablets group.Conclusion Compound Lishao Tablets can exert therapeutic effects on primary trigeminal neuralgia,and its mechanism may be related to reducing the expression of inflammation related factors and proteins.
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