Determination of chlorogenic acid,glycyrrhizin,luteolin,forsythiaside A,forsythin,and rhein in Lianhua Qingwen Capsules by HPLC-quantitative analysis of multi-components by single marker
Objective To establish an HPLC-quantitative analysis of multi-components by single marker(QAMS)to determine contents of chlorogenic acid,glycyrrhizin,luteolin,forsythiaside A,forsythin,and rhein in Lianhua Qingwen Capsules.Methods Analysis was performed on an Inertsil ODS-3 column(250 mm×4.6 mm,5 μm).The mobile phase consisted of acetonitrile-0.4%phosphoric acid aqueous in gradient mode.The flow rate was 1.0 mL/min,the column temperature was maintained at 25℃,and the detection wavelength was set at 280 nm.Using forsythin as the internal reference substance,the relative correction factor of the other five components was established,and the contents of each component were calculated by QAMS method.Results Chlorogenic acid,glycyrrhizin,luteolin,forsythiaside A,forsythin,and rhein all showed good linear relationship within the ranges of 0.243—4.861,0.112—2.248,0.087—1.732,0.247—4.949,0.370—7.402,and 0.095—0.952 μg/mL(r≥0.999 5),and their average recovery rates were 99.40%,101.0%,104.5%,98.17%,99.70%,and 99.58%,with the RSD of 1.12%,2.53%,3.15%,2.94%,1.41%,and 2.84%(n = 6).Conclusion This method has a high recovery rate and good repeatability,providing a new method for establishing a more comprehensive quality control of Lianhua Qingwen Capsules.
Lianhua Qingwen Capsuleschlorogenic acidglycyrrhizinluteolinforsythiaside AforsythinrheinHPLCquantitative analysis of multi-components by single marker method
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