Effects of asiaticoside on Nrf2 and ROS pathway expression in rats with metabolically associated fatty liver disease
Objective To investigate the effects of asiaticoside on Nrf2 and ROS pathway expression in rats with metabolically associated fatty liver disease.Methods Rats were randomly divided into control group,model group,asiaticoside(5,15,30 mg/kg)group,and bicyclic alcohol group,with 10 rats in each group.Control group and model group were ig 10 mL/kg normal saline,asiaticoside(5,15,30 mg/kg)group were ig 5,15,30 mg/kg asiaticoside,bicyclic alcohol group were ig 20 mg/kg bicyclic alcohol group,once daily for 4 weeks.ELISA was used to detect ALT,AST,TC,and TG,fluorescent probe dihydroethidine(DHE)method was used to detect ROS,and HE staining was used to observe the pathological tissue.The relative expression levels of Nrf1 and Nrf2 protein and mRNA were detected by Western blotting and PCR respectively.Results Compared with model group,ALT,AST,TC,TG and ROS in 15 and 30 mg/kg asiaticoside groups were significantly decreased,while Nrf1 and Nrf2 mRNA and protein were significantly increased(P<0.05).The liver structure of control group was intact.The hepatocyte steatosis of 15,30 mg/kg asiaticoside group and dicyclol group was reduced,and a small amount of inflammatory cell infiltration was observed in the sink area.Conclusion Asiaticoside can reduce the levels of ALT,AST,TC,and TG in serum of rats with metabolism-related fatty liver disease,and inhibit the growth of ROS,which may be related to the activation of Nrf1 and Nrf2.
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