Objective To investigate the bacteriostatic effect of Lactobacillus plantarum RG-034 in vitro and its therapeutic effect on the diarrhoea-predominant irritable bowel syndrome in rats.Methods The viable bacteria count of RG-034 powder was determined by using the"Oxford Cup method"to determine the antibacterial activity of Lactobacillus plantarum RG-034 against Escherichia coli,Salmonella paratyphi B and Staphylococcus aureus.SD rats were randomly divided into control group,model group,Montmorillonite Powder group,and Lactobacillus plantarum RG-034 capsule group by body weight,with 6 rats in each group.Except the control group,other rats were given 5 g/kg senna leaf combined with alternate day fasting daily for 5 weeks to prepare a rat model of diarrhea-type irritable bowel syndrome.At the 3rd week of self-modeling,rats in Lactobacillus plantarum RG-034 group were given RG-034 Capsules(1×109 CFU/Capsule,15 mg),2 capsules/d.Rats in Montmorillonite Powder group were ig 0.81 g/kg Montmorillonite Powder once daily.The rats in control group and model group were given the same amount of normal saline intragaically for 3 weeks.The changes of the general status of rats were observed,the rate of loose stool,the grade of loose stool and the diarrhea index were determined,and the changes of intestinal flora in the colon of rats were analyzed by 16S ribosomal RNA sequencing technology.Results The viable bacteria number of Lactobacillus plantarum RG-034 Powder was 2.5×1011 CFU/g,which had certain inhibitory effect on Escherichia coli,Salmonella paratyphi B and Staphylococcus aureus at different concentrations,and the inhibitory zone diameter was 15.90—25.06 mm.Compared with the model group,Lactobacillus plantarum RG-034 Capsules can significantly improve the diarrhea situation of rats,significantly reduce the rate of loose stool,loose stool grade and diarrhea index,and increase the diversity of intestinal flora.Conclusion Lactobacillus plantarum RG-034 has a certain therapeutic effect on diarrhea-predominant irritable bowel syndrome in rats,and its mechanism may be related to the regulation of intestinal microbiota diversity.
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