Quality evaluation of Fici Pumilae Receptaculum by UPLC fingerprint combined with multiple index components
Objective To evaluate the crude drug of Fici Pumilae Receptaculum from different origins by characteristic chromatogram and multiple index.Methods UPLC was used to establish the characteristic chromatogram and the determination of chlorogenic acid and rutin in Fici Pumilae Receptaculum,and the quality evaluation model of Fici Pumilae Receptaculum was established by using the entropy weight method as the resolution coefficient.The correlation between various indicators was calculated by grey relation analysis.Results Eight common peaks were established in UPLC characteristic chromatogram,and that 3 of them were identified as neochlorogenic acid,chlorogenic acid,and cryptochlorogenic acid.Contents of chlorogenic acid and rutin in 13 batches of Fici Pumilae Receptaculum from different origins were determined.The relative correlation degree of 13 batches of samples ranged from 0.116 2 to 0.810 5.There were certain differences in quality between different batches of samples,and Fici Pumilae Receptaculum samples from Zhaoqing City in Guangdong Province was better.Conclusion The method can effectively evaluate the quality of Fici Pumilae Receptaculum from different origins,providing reference for the quality control of Fici Pumilae Receptaculum crude drug.
Fici Pumilae Receptaculumcharacteristic chromatogramchlorogenic acidrutinentropy weight methodgray correlation methodUPLC
NETL
NSTL
万方数据
