Impact of butorphanol on nerve cell inflammation in rats with spinal cord injury by regulating cGAS/STING signal pathway
Objective To investigate the impact of butorphanol on nerve cell inflammation in rats with spinal cord injury and its mechanism.Methods Spinal cord injury rat models were duplicated and grouped into sham operation group,model group,butorphanol(100 μg/kg)group,and butorphanol + 2',3'-cGAMP(100 μg/kg butorphanol + 500 μg/kg 2',3'-cGAMP)group,the rats were scored for motor function,HE staining was applied to detect the pathological changes of spinal cord tissue,TUNEL staining was applied to detect apoptosis,the levels of TNF-α,IL-1β,and IL-18 in spinal cord tissue were detected by ELISA,immunofluorescence staining was applied to detect microglia,Western blotting was applied to detect the expression of cGAS,STING,NLRP3,Caspase-1,and IL-1β proteins in spinal cord tissue.Results Compared with model group,the injury of spinal cord tissue,inflammatory cells and necrotic cells were reduced in butorphanol group,BBB score was obviously higher,the apoptosis rate of spinal cord tissue,the levels of microglia and TNF-α,IL-1β,IL-18,and the protein expression of cGAS,STING,NLRP3,Caspase-1,and IL-1β were obviously decreased(P<0.05).Compared with butorphanol group,the spinal cord tissue injury in butorphanol + 2',3'-cGAMP group was aggravated,the cell arrangement was loose,and inflammatory cells were increased,the BBB score was obviously lower,the apoptosis rate of spinal cord tissue,the levels of microglia and TNF-α,IL-1β,IL-18,and the protein expression of cGAS,STING,NLRP3,Caspase-1,and IL-1β were obviously higher(P<0.05).Conclusion Butorphanol can improve spinal cord injury in rats by inhibiting the activation of cGAS/STING signal pathway and inhibiting the activation of microglia and inflammatory reaction.
butorphanolspinal cord injuryneuroinflammationcGAS/STING signal pathwayTNF-αNLRP3Caspase-1
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