Analysis on mechanism of Xueping Tablets in treatment of irregular menstruation based on network pharmacology and molecular docking technique
Objective To investigate the mechanism of Xueping Tablets in treatment of irregular menstruation based on network pharmacology and molecular docking technique.Methods TCMSP database was used to search the active targets of the active ingredients of Xueping Tablets,GeneCard and Disgenet databases were used to search the disease targets related to"irregular menstruation",and the intersecting targets were screened and inputted into the String database to construct a protein-pathway-target interactions network.The disease-pathway-target-chemical composition drug network was constructed by analyzing and screening the core targets using Cypscape database.The core targets were analyzed and screened by Cypscape database,and the disease-pathway-target-chemical component drug network was constructed.The intersecting targets were analyzed by David database for gene ontology(GO)function and KEGG pathway enrichment.Results Naringenin,rhubarb phenol,kaempferol,rubiadin,rhodopsin and rhodopsin methyl ether were screened by network topology mapping for their association with serine threonine specific protein kinase(AKT1),tumor necrosis factor(TNF),proto-oncogene tyrosine protein kinase(SRC),estrogen receptor 1(ESR1),epidermal growth factor receptor(EGFR),peroxisome.The molecular docking validation of the first six core targets of peroxisome proliferator-activated receptor γ(PPARG)showed that rhodopsin,rubiadin,kaempferol and other active ingredients of blood leveling tablets had better binding activities with AKT1,TNF,SRC,ESR1,EGFR and PPARG.Conclusion Xueping Tablets can treat irregular menstruation through multi-component action and multi-target.
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