Identification on components in Wuzhi Capsules and rats blood components by UPLC-Q-TOF-MS method
Objective To identify the components in Wuzhi Capsules and rats blood components based on serum pharmacochemistry technology.Methods Samples of ingredients of Wuzhi Capsules group,Wuzhi Capsules group,and blank group were analyzed by UPLC-Q-TOF-MS method.The data were analyzed by MassLynx V4.1 software,and the components in Wuzhi Capsules and rats blood components were identified.Results There were 15 identified components in in Wuzhi Capsules,and the content of schisandrin A was the highest.The serum of rats contained two prototype components in Wushizi Capsules,namely schisantherin D and changnanic acid,and 8 metabolites were screened out.These components were mainly composed of hydrolysis,oxidation,and demethoxylation reaction in phase I metabolic reactions,as well as glucuronidation in phase II metabolic reaction.Conclusion The method can identify active ingredients and metabolites in Wuzhi Capsules,providing a basis for clinical application of Wushizi Capsules.
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