UPLC-Q-TOF-MS法鉴定五酯胶囊中化学成分和大鼠入血成分
Identification on components in Wuzhi Capsules and rats blood components by UPLC-Q-TOF-MS method
王磊 1李嘉晨 1刘洪庚 1刘莹 1孙悦 1祝逸飞 2王玉明2
作者信息
- 1. 天津市第五中心医院生态城医院,天津 300467
- 2. 天津中医药大学,天津 301617
- 折叠
摘要
目的 基于血清药物化学技术对五酯胶囊中成分和大鼠入血成分进行鉴定.方法 采用UPLC-Q-TOF-MS法对五酯胶囊成分组、给药组和空白组样品进行分析,通过MassLynx V4.1 软件对所得图谱进行数据解析,对五酯胶囊中成分及其入血成分进行鉴定.结果 鉴定出五酯胶囊中 15 个化合物,其中五味子甲素含量最高.大鼠血清中含有五酯胶囊 2 个原型成分五味子酯丁、长南酸,筛选得到代谢物 8 个,主要以I相代谢反应中的水解、氧化、去甲氧基化反应和II相代谢反应中的葡萄糖醛酸化结合为主.结论 建立了五酯胶囊中活性成分和代谢产物的鉴定方法,为五酯胶囊的临床应用提供依据.
Abstract
Objective To identify the components in Wuzhi Capsules and rats blood components based on serum pharmacochemistry technology.Methods Samples of ingredients of Wuzhi Capsules group,Wuzhi Capsules group,and blank group were analyzed by UPLC-Q-TOF-MS method.The data were analyzed by MassLynx V4.1 software,and the components in Wuzhi Capsules and rats blood components were identified.Results There were 15 identified components in in Wuzhi Capsules,and the content of schisandrin A was the highest.The serum of rats contained two prototype components in Wushizi Capsules,namely schisantherin D and changnanic acid,and 8 metabolites were screened out.These components were mainly composed of hydrolysis,oxidation,and demethoxylation reaction in phase I metabolic reactions,as well as glucuronidation in phase II metabolic reaction.Conclusion The method can identify active ingredients and metabolites in Wuzhi Capsules,providing a basis for clinical application of Wushizi Capsules.
关键词
五酯胶囊/五味子甲素/五味子酯丁/长南酸/代谢物/UPLC-Q-TOF-MS法Key words
Wuzhi Capsules/schisandrin A/schisantherin D/changnanic acid/metabolite/UPLC-Q-TOF-MS引用本文复制引用
出版年
2024
NETL
NSTL
万方数据