HPLC多指标成分定量结合化学计量学、加权TOPSIS模型评价不同产地肺形草质量
Evaluation of Tripterospermum chinense from different origins by HPLC multi index component combined with chemometrics and weighted TOPSIS model
张立群 1马诗瑜 1冯恺 2徐虹1
作者信息
- 1. 上海交通大学医学院附属瑞金医院 药剂科,上海 200025
- 2. 上海中医药大学,上海 200032
- 折叠
摘要
目的 评价不同产地肺形草的质量差异.方法 采用HPLC法测定肺形草中马钱苷酸、莫诺苷、獐牙菜苦苷、龙胆苦苷、芒果苷、异荭草苷、异牡荆素、去甲基雏菊叶龙胆酮、当药黄素、3,6,8-三羟基-1-甲基呫吨酮、山楂酸和熊果酸,对多指标成分测定结果进行主成分分析、正交偏最小二乘法-判别分析,建立加权优劣解距离模型评估.结果 16 批肺形草明显聚集为 3 类;马钱苷酸、莫诺苷、3,6,8-三羟基-1-甲基呫吨酮、熊果酸、当药黄素和芒果苷是影响肺形草质量的主要潜在标志物;浙江和江苏产肺形草整体质量较好.结论 建立的方法控制模式简单、评价模型科学合理,可用于肺形草质量评价.
Abstract
Objective To evaluate the quality difference of Tripterospermum chinense from different origins.Methods Contents of loganic acid,morroniside,swertiamain,gentiopicrin,mangiferin,isoorientin,isovitexin,demethylbellidifolin,swertisin,3,6,8-trihydroxy-1-methyl-xanthen-9-one,maslinic acid and ursolic acid in T.chinense were determined by HPLC method.PCA and OPLS-DA were used to analyze the contents of multi-component components,and the weighted-TOPSIS model was established to evaluate the quality.Results 16 Batches of T.chinense were clustered into three categories,and loganic acid,morroniside,3,6,8-trihydroxy-1-methyl-xanthen-9-one,ursolic acid,swertisin,and mangiferin were the main potential markers affecting the quality of T.chinense.The overall quality of T.chinense from Zhejiang and Jiangsu Provinces is good.Conclusion The established method has a simple control mode and a scientifically reasonable evaluation model,which can be used for the quality evaluation of T.chinense.
关键词
肺形草/钱苷酸/莫诺苷/獐牙菜苦苷/龙胆苦苷/芒果苷/异荭草苷/异牡荆素/去甲基雏菊叶龙胆酮/当药黄素/3,6,8-三羟基-1-甲基呫吨酮/山楂酸/熊果酸/高效液相色谱/主成分分析/正交偏最小二乘法-判别分析/加权优劣解距离模型Key words
Tripterospermum chinense(Migo)H.Smith/loganic acid/morroniside/swertiamain/gentiopicrin/mangiferin/isoorientin/isovitexin/demethylbellidifolin/swertisin/3,6,8-trihydroxy-1-methyl-xanthen-9-one/maslinic acid/ursolic acid/HPLC/PCA/OPLS-DA/weighted-TOPSIS mod引用本文复制引用
基金项目
上海市"医苑新星"青年医学人才培养资助计划(沪卫人事[2022]65号)
出版年
2024
NETL
NSTL
万方数据