Evaluation of Tripterospermum chinense from different origins by HPLC multi index component combined with chemometrics and weighted TOPSIS model
Objective To evaluate the quality difference of Tripterospermum chinense from different origins.Methods Contents of loganic acid,morroniside,swertiamain,gentiopicrin,mangiferin,isoorientin,isovitexin,demethylbellidifolin,swertisin,3,6,8-trihydroxy-1-methyl-xanthen-9-one,maslinic acid and ursolic acid in T.chinense were determined by HPLC method.PCA and OPLS-DA were used to analyze the contents of multi-component components,and the weighted-TOPSIS model was established to evaluate the quality.Results 16 Batches of T.chinense were clustered into three categories,and loganic acid,morroniside,3,6,8-trihydroxy-1-methyl-xanthen-9-one,ursolic acid,swertisin,and mangiferin were the main potential markers affecting the quality of T.chinense.The overall quality of T.chinense from Zhejiang and Jiangsu Provinces is good.Conclusion The established method has a simple control mode and a scientifically reasonable evaluation model,which can be used for the quality evaluation of T.chinense.
Tripterospermum chinense(Migo)H.Smithloganic acidmorronisideswertiamaingentiopicrinmangiferinisoorientinisovitexindemethylbellidifolinswertisin3,6,8-trihydroxy-1-methyl-xanthen-9-onemaslinic acidursolic acidHPLCPCAOPLS-DAweighted-TOPSIS mod