Ameliorating effect of cannabidiol on imiquimote-induced psoriasis in mice
Objective To investigate the therapeutic effect of cannabidiol on imiquimod-induced psoriatic inflammation and oxidative stress index in mice.Methods BALB/c mice were randomly assigned to control group,model group,tacrolimus group,and cannabidiol(50,100,150 mg/kg)groups,with 8 mice in each group.In addition to the control group,62.5 mg of 5%imiquimot-induced psoriasis model was evenly applied on the back of mice in other groups every day.4 h after modeling,tacrolimus group was evenly applied with Tacrolimus Cream,cannabidiol group was evenly applied with 50,100,150 mg/kg cannabidiol alcohol solution,control group and model group were evenly applied with petroleum jelly on the back.PASI score was recorded by taking photos once daily for 7 consecutive days.The contents of IL-6 and TNF-α in skin tissue were detected by enzyme-linked immunosorbent assay 24 h after the last administration.HE staining of skin tissue;the spleen of mice was weighed,and the spleen index was calculated.The activity of SOD and the contents of GSH,and MDA in the skin of mice were detected.Results PASI score and HE staining results showed that,compared with model group,cannabidiol group could significantly improve the characteristics of psoriasis such as redness,thickening and increased dander of the back skin of mice,and the epidermal thickening of the back skin of mice was significantly reduced.The spleen index was significantly decreased(P<0.05,0.01).In skin tissue samples,the contents of IL-6 and TNF-α in cannabidiol group were significantly decreased compared with model group(P<0.01,0.001).Cannabidiol group could significantly increase SOD activity and GSH content in the skin tissue of psoriasis mice,and decrease MDA content(P<0.01,0.001).Conclusion Cannabidiol significantly inhibited the psoriatic inflammation induced by imiquimod in mice,and inhibited the infiltration of inflammatory cells and the production of cytokines such as oxidative stress state.
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