Effect of glaucocalyxin B on the growth of cervical cancer transplanted tumor in nude mice through Akt/BAD pathway
Objective To investigate the impact and mechanism of glaucocalyxin B on the growth of cervical cancer transplanted tumor in nude mice through the Akt/Bcl-2 associated BAD pathway.Methods 48 SPF grade female Balb/c nu nude mice were randomly grouped into model group,glaucocalyxin B(56,112 mg/kg),and glaucocalyxin B+SC79(112 mg/kg+10 mg/kg)group,with 12 mice in each group.HeLa cells were implanted subcutaneously at the dorsal side of the right thigh root in nude mice to establish a subcutaneous transplanted tumor model,and the changes in tumor volume,weight,and general situation were observed.HE staining was applied to observe the pathological changes of tumor tissue.TUNEL assay was applied to detect the apoptosis of tumor histiocyte.Western blotting method was applied to detect the expression levels of p-Akt,Akt,p-BAD,BAD,and PTEN proteins in transplanted tumor tissue.Results Compared with the model group,the tumor volume at 22,25,and 28 day,tumor weight,Akt,and BAD phosphorylation levels in tumor tissue of the glaucocalyxin B group were obviously decreased(P<0.05),the apoptosis rate of tumor cells and the expression of PTEN protein in tumor tissue were obviously increased(P<0.05).SC79 could weaken the inhibitory effect of glaucocalyxin B on the growth of cervical cancer transplanted tumor in nude mice.Conclusion Glaucocalyxin B inhibited the growth of transplanted tumors in nude mice with cervical cancer,and its mechanism of action was related to the inhibition of the Akt/BAD signaling pathway.
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