Mechanism of rosmarinic acid in treatment of hyperuricemia based on TMT proteomics technology
Objective To investigate the mechanism of rosmarinic acid against hyperuricemia in rats by TMT proteomics.Methods SD rats were randomly divided into control group,model group,benzbromarone group,and rosmarinic acid(50 and 100 mg/kg)group.Control group was given distilled water,and the other groups were given 10%fructose water to establish the model of hyperuricemia.After 4 weeks of continuous administration,the serum uric acid level,XOD and ADA activity were detected,and the pathological changes of ileum tissue were observed by HE staining.RT-qPCR was used to detect the gene expression of uric acid transporter ABCG2 and GLUT9 mRNA in the ileum tissue.TMT proteomics was used to detect the protein expression in the ileum tissue of rats.Results Compared with the model group,rosmarinic acid significantly reduced the serum uric acid level,improved XOD and ADA activities(P<0.001,0.05,0.01),and improved the expression of GLUT9 mRNA in the ileum tissue(P<0.05).A total of 2 975 differentially expressed proteins were screened by TMT proteome sequencing(log2FC>1.5,P<0.05).Pathway analysis showed that rosmarinic acid mainly regulated Th1/2/17 cell differentiation and other pathways.Conclusion Mechanism of rosmarinic acid against hyperuricemia may be related to the inhibition of ADA and XOD activities,regulation of intestinal protein GLUT9 mRNA expression and Th1/2/17 cell differentiation pathway.
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