Mechanism of quercetin in treatment of intrauterine adhesion based on network pharmacology and macromolecular docking
Objective To study on the mechanism of quercetin in treatment of intrauterine adhesion by using network pharmacology and macromolecular docking.Methods The target of quercetin was found through TCMSP,the target of intrauterine adhesion was obtained through GeneCards,OMIM,DrugBank,and PharmGKB.After taking the intersection target of quercetin and intrauterine adhesion,a protein-protein interaction network was constructed using the STRING database,the core targets were obtianed based on topological parameter analysis.The core targets were used for GO functional enrichment analysis and KEGG pathway enrichment analysis by the Metascape platform and microbiological information platform.Finally,macromolecular docking was used to verify the binding ability between quercetin and the core targets.Results There are 105 common targets of quercetin and uterine adhesion,among which the core targets are HIF-1α,Akt1,MAPK1,JUN,etc.The main biological processes involved in the treatment of uterine adhesion by quercetin include bacterial response,lippolysaccharide response,oxidative stress and reactive oxygen metabolism,etc.The key pathways are PI3K/Akt signaling pathway,TNF signaling pathway,and IL-17 signaling pathway.The results of molecular docking showed that quercetin had the best docking effect with HIF-1α.Conclusion Quercetin acts on HIF-1α,Akt1,MAPK1,and other key targets,participates in the regulation of PI3K/Akt,TNF,IL-17 and other signaling pathways,inhibits inflammatory response and oxidative stress,and thus plays a role in the treatment of uterine adhesions.
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