Objective To observe effects of Erxiekang Plaster on functional abdominal pain juvenile rats,and explore its mechanism.Methods The 1-day-old SD rats were randomly divided into control group and model group.The functional abdominal pain rat models with visceral hypersensitivity were established by the method of combination of maternal separation and ice water stress.The rats with successful modeling were randomly divided into model group,Dingguier Umbilical Paste group,and Erxiekang Plaster(1/24,1/12,1/6)group.Each group of rats was given abdominal patches for 7 consecutive days,while the control group and model group were given blank simulated patches on the abdomen.Scores of Bristol classification and water content of the fecal pellets and abdominal withdrawal reflex(AWR)of rats in all the groups were observed after treatment.ELISA was used to detect the changes of 5-HT,SP and TNF-α in serum and colon tissue.The mRNA expression levels of CFTR and CRF in colonic tissues were detected by RT-PCR.The protein expression levels of both were detected by Western blotting.Results Compared with the model group,the Bristol score and fecal water content of rats significantly decreased after treatment with Erxiekang Plaster,and the AWR score significantly decreased under different water injection volumes.The expression levels of 5-HT,SP,and TNF-α in serum and colon tissue were significantly decreased.The CFTR and CRF mRNA and protein expression significantly down-regulated.Conclusion Erxiekang Plaster has a significant therapeutic effect on functional abdominal pain in rats.It can significantly reduce the Bristol score and fecal water content,as well as visceral sensitivity of rats by regulating the content of abdominal pain and diarrhea related factors in serum and colon tissue,as well as the expression of related proteins in colon tissue.
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