Objective To investigate the effects of Wuzi Yanzong Pills on spermatogenesis,expression of inositol hexaphosphate kinase 1(IP6K1)and methylation of mitochondrial DNA(mtDNA)in oligoasthenospermia mice.Methods Male BALB/c mice were randomly divided into control group,model group,and Wuzi Yanzong Pills(1,2,and 4 g/kg)group,with 10 mice in each group.Except the control group,the other groups were given 40 mg/kg intraperitoneal injection.Starting from the second day of model making,each group was given the drug by intragathy according to dose.After 4 weeks of administration,the coefficients of testis and epididymis organs were calculated,the sperm number and vitality were detected,the histopathological changes of testis were observed by HE staining,and the apoptosis of testicle cells was observed by TUNEL staining.The expression of IP6K1 was detected by RT-qPCR,Western blotting and immunohistochemistry(IHC).The expression of DNA methyltransferase 1(DNMT1)was detected by Western blot,the D-loop methylation of mtDNA was detected by pyrosequencing,and the mitochondrial gene expression was detected by RT-qPCR.Results Wuzi Yanzong Pills could partially restore the testicular and epididymal coefficients of oligoasthenospermia mice,significantly increase sperm count and motility,significantly improve testicular histopathological injury and spermatogenic cell apoptosis,and significantly promote the expression of IP6K1.DNMT1 expression and mitochondrial D-loop methylation levels were significantly inhibited,and the expression of mitochondrial coding genes was significantly up-regulated(P<0.5,0.1,0.001).Conclusion Wuzi Yanzong Pills can improve the spermatogenic function of oligoasthenospermia mice,and its mechanism may be related to promoting the expression of IP6K1,inhibiting DNMT1/mtDNA D-loop methylation,and promoting the expression of mitochondrial coding genes.
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