Objective To identify the chemical constituents in Potentillae Radix by UPLC-QTOF/MS.Methods The separation was carried out on Acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm)with the mobile phase of 0.05%formic acid-acetonitrile by gradient elution.The flow rate was 0.3 mL/min,column temperature was 40℃,and the volume of sample injection was 2 μL.Electrospray ionization(ESI)was used.Positive and negative ion scanning of ESI electric spray ion source was used,and fragmented data were collected under the second level IDA scanning mode.Identification of chemical components in Potentillae Radix water extract were speculated using Sciex OS 2.0 data processing software combined with databases,reference substances,some literature,and natural product cleavage pathways.Results A total of 53 chemical constituents were identified,including 10 amino acids,8 phenolic acids,4 terpene acids,7 flavonoids,1 quaternary amine alkaloid,and 23 others.The fragmentation process of fragment ions was speculated taking gallic acid and tannic acid as example.Conclusion The method is accurate,stable,and suitable for identification of chemical constituents in Potentillae Radix,and provides reference for subsequent quality control and material basis research.
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