UPLC法测定小柴胡颗粒中柴胡皂苷b1、b2
Determination of saikosaponin b1 and saikosaponin b2 in Xiaochaihu Granules by UPLC
王培 1许保海 1孙逸轩 2刘长利2
作者信息
- 1. 首都医科大学附属北京积水潭医院 中药房,北京 100035
- 2. 首都医科大学 中医药学院,北京 100069
- 折叠
摘要
目的 建立UPLC法测定小柴胡颗粒中柴胡皂苷b1、b2.方法 使用Acquity UPLC BHE C18 色谱柱(100 mm×2.1 mm,1.7 μm);流动相:水-乙腈,梯度洗脱;检测波长:254 nm;体积流量:0.3 mL/min;柱温:30℃;进样量3 μL.结果 柴胡皂苷b1、b2 线性范围分别为 0.006～0.374 mg、0.003～0.214 mg,平均回收率分别为 98.97%、99.35%,RSD值分别为 1.82%、1.16%.结论 所建立方法准确、稳定,重现性好,可用于小柴胡颗粒的质量控制和评价.
Abstract
Objective To develop an UPLC method for determination the saikosaponin b1 and saikosaponin b2 in Xiaochaihu Granules.Methods The analysis was applied on Acquity UPLC BHE C18 column(100 mm×2.1 mm,1.7 μm).The mobile phase consisted of water and acetonitrile with gradient elution.The detection wavelength was set at 254 nm,flow rate was 0.3 mL/min,column temperature was 30℃,and volume of injection was 3 μL.Results Saikosaponin b1 and saikosaponin b2 showed good linear relationships of 0.006—0.374 mg and 0.003—0.214 mg,whose average recoveries were 98.97%and 99.35%with RSD values of 1.82%and 1.16%.Conclusion The established method is accurate,stable,and has good reproducibility,and can be used for quality control and evaluation for Xiaochaihu Granules.
关键词
小柴胡颗粒/柴胡皂苷b1/柴胡皂苷b2/超高效液相色谱Key words
Xiaochaihu Granules/saikosaponin b1/saikosaponin b2/UPLC引用本文复制引用
基金项目
北京市中医药管理局第三批中药骨干人才培养项目(京中医科字[2022]59号)
出版年
2024
NETL
NSTL
万方数据