Objective To develop an UPLC method for determination the saikosaponin b1 and saikosaponin b2 in Xiaochaihu Granules.Methods The analysis was applied on Acquity UPLC BHE C18 column(100 mm×2.1 mm,1.7 μm).The mobile phase consisted of water and acetonitrile with gradient elution.The detection wavelength was set at 254 nm,flow rate was 0.3 mL/min,column temperature was 30℃,and volume of injection was 3 μL.Results Saikosaponin b1 and saikosaponin b2 showed good linear relationships of 0.006—0.374 mg and 0.003—0.214 mg,whose average recoveries were 98.97%and 99.35%with RSD values of 1.82%and 1.16%.Conclusion The established method is accurate,stable,and has good reproducibility,and can be used for quality control and evaluation for Xiaochaihu Granules.
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