Exploring effect and mechanism of Periplaneta americana extract CⅡ-3 to induce senescence of ovarian cancer SKOV3 cells based on the PI3K/Akt/FoxO4 signaling pathway
Objective To investigate the anti-tumour effects and potential mechanisms of the Periplaneta americana extract CⅡ-3 on ovarian cancer SKOV3 cells. Methods In vitro,SKOV3 cells were cultured and divided into control group and CⅡ-3 group,and the proliferative capacity of SKOV3 cells was determined by the Cell Counting Kit-8 (CCK-8) and the cell colony formation assay. Flow cytometry was used to detect the changes in the cell cycle and mitochondrial membrane potential of SKOV3 cells. Positive senescence-associated beta-galactosidase (SA-β-Gal) staining assay to detect senescence in SKOV3 cells. q-PCR was utilized to assess the modification of telomerase reverse transcriptase (TERT),and PI3K,Akt,FoxO4 mRNA in SKOV3 cells. Western blotting assays were conducted to determine the expression of p-PI3K,p-Akt,p-FoxO4 protein in cells. Results Compared with the control group,Periplaneta americana extract CⅡ-3 exhibited a significant inhibitory effect on the proliferation of SKOV3 cells,accompanied by a notable reduction in colony-forming ability,an increase in the proportion of cells arrested in the G0/G1 phase. The mitochondrial membrane potential decreased,the positive rate of senescence of SA-β-Gal cells increased,the activity of TERT decreased,the expression of PI3K,Akt mRNA and p-PI3K,p-Akt protein expression were up-regulated,and both FoxO4 mRNA and p-FoxO4 protein expression were down-regulated. Conclusion Periplaneta americana extract CⅡ-3 induced cellular senescence in SKOV3 cells by regulating the PI3K/Akt/FoxO4 signaling pathway.
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