高效液相色谱-示差折光检测器法测定牛磺酸颗粒中牛磺酸
Determination of taurine in Taurine Granules by HPLC-RID
覃婷婷 1房思萌 1王培 1鲁鑫 1白海娇 1韩晓捷1
作者信息
- 1. 天津市药品检验研究院,天津 300070
- 折叠
摘要
目的 建立高效液相色谱-示差折光检测器(HPLC-RID)法测定牛磺酸颗粒中牛磺酸.方法 采用Welch Ultimate HILIC Amide色谱柱(250 mm×4.6 mm,5μm),以0.1％冰醋酸-乙腈(30∶70)为流动相,柱温为30℃,示差折光检测器温度30℃,体积流量1.0 Ml/min,进样体积20μL.结果 牛磺酸在0.008～1.211 mg/Ml与峰面积呈良好线性关系,平均回收率为99.9％,RSD值为0.7％.结论 本法测定准确、可靠、专属性强,为牛磺酸制剂的测定提供新的检测方法.
Abstract
Objective To establish an HPLC-RID method for determination of taurine in Taurine Granules. Methods Welch Ultimate HILIC Amide column (250 mm × 4.6 mm,5 μm) was used. The mobile phase was 0.1％ glacial acetic acid-acetonitrile (30∶70). The column temperature was 30 ℃,and the differential refractive detector temperature was 30 ℃. The volume flow rate was 1.0 Ml/min,and the injection volume was 20 Μl. Results Taurine had good linear relationships of 0.008-1.211 mg/Ml,and the average recovery rate was 99.9％ with RSD 0.7％. Conclusion This method is accurate,reliable,and highly specific,which provides a new detection method for the determination of taurine preparations.
关键词
牛磺酸颗粒/牛磺酸/HPLC-RIDKey words
Taurine Granules/taurine/HPLC-RID引用本文复制引用
基金项目
国家药品标准制修订研究课题(2021H032)
出版年
2024
NETL
NSTL
万方数据