Impacts of baicalein on blood-brain barrier,neuronal pyroptosis and NLRP3/Caspase-1 signaling pathway in rats with traumatic brain injury
Objective To investigate the impacts of baicalein on the blood-brain barrier and neuronal pyroptosis in rats with traumatic brain injury,and its impact on the NLRP3/Caspase-1 signaling pathway.Methods Rats were assigned into control group,model group,baicalein(2.5,5.0,and 10.0 mg/kg)group,with 12 rats in each group.Baicalein groups were intraperitoneally injected with baicalein at doses of 2.5,5.0,and 10.0 mg/kg,respectively.The serum TNF-α,IL-6,GSH-Px,SOD,and MDA were determined by ELISA method.The superfluid of Evans blue(EB)dye was used to measure the permeability of the blood-brain barrier.TUNEL and Caspase-1 double staining method was applied to detect cell apoptosis.RT-qPCR was applied to measure the mRNA expression levels of ZO-1,Claudin-1,and Occludin in hippocampal tissue.The expression of NLRP3/Caspase-1 pathway related proteins in hippocampal tissue were detected by Western blotting.Results Compared with the model group,the EB content in the affected hemisphere of rats in the baicalein groups were greatly reduced,and the permeability of the blood-brain barrier was greatly improved,the levels of ZO-1,Claudin-1,Occludin mRNA,and SOD and GSH-Px were greatly increased(P<0.05),the cell apoptosis rate and the levels of MDA,IL-6,TNF-α,NLRP3,Caspase-1,GSDMD-N,IL-1β,and IL-18 were greatly decreased(P<0.05).Conclusion Baicalein can inhibit the NLRP3/Caspase-1 signaling pathway,alleviate inflammatory and oxidative stress responses,and improve the blood-brain barrier and neuronal pyroptosis in traumatic brain injury rats.
baicaleintraumatic brain injuryNOD-like receptor thermal protein domain associated protein 3Caspase-1oxidative stressinflammatory reaction
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