Ziyuglycoside Ⅱ alleviates diabetic retinopathy in diabetes in vitro and in vivo based on PI3K/Akt/mTOR pathway
Objective To explore the effect of ziyuglycoside Ⅱ on diabetic retinopathy in streptozotocin-induced rats based on PI3K/Akt/mTOR pathway.Methods Rat model of diabetic retinopathy was established by intraperitoneal injection of streptozotocin 35mg/kg.Cell model of diabetic retinopathy was established by glucose-induced Müller cell.The levels of cytokines in serum,retina and cell supernatant were detected by ELISA.Serum insulin and OGTT,serum SOD activity and MDA content were measured by the kit.The pathological changes of retinal tissue were evaluated by HE staining.Western blotting was used to detect the PI3K/Akt/mTOR signaling pathway in retinal tissues of diabetic mice and Müller cells induced by high glucose.Results Compared with model group,ziyuglycoside Ⅱ significantly decreased serum insulin level and improved OGTT.SOD levels in serum,retina and Müller cells were significantly increased and MDA levels were decreased.ziyuglycoside Ⅱ also significantly reduced serum and retinal cytokines in diabetic rats and high-sugar-induced Müller cells.Conclusions Ziyuglycoside Ⅱ could inhibited PI3K/Akt/mTOR signal pathway in retina tissue and glucose-induced Müller cell.ziyuglycoside Ⅱ may improve retinopathy in diabetic retinopathy,oxidative stress and inflammation in the retina tissue of rats and glucose-induced Müller cell.
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