Mechanism of melezitose in treatment of liver fibrosis based on network pharmacology and experimental verification
Objective To investigate the mechanisms of the melezitose in treating liver fibrosis using network pharmacology and molecular docking techniques,and to validate the core targets.Methods The related targets of melezitose in treatment of liver fibrosis were screened from the Swiss Target Prediction database.The related targets of liver fibrosis were screened from OMIM,GeneCards and Disgenet databases.The protein interaction network was carried out on the STRING database,and the tsv format data results were imported into Cytoscape 3.9.1 software for visual analysis.DAVID database was further used for GO analysis and KEGG enrichment analysis,and molecular docking.The viability of mouse hepatic stellate cell line JS-1 was measured by MTT assay.Western blotting verified the protein expression level of the core target.Results KEGG enrichment analysis showed that the intersection targets involved 64 signaling pathways,mainly PI3K/Akt and MAPK signaling pathways.Molecular docking suggested that pine triose could bind well to the core target.Western blotting results showed that the phosphorylated PI3K/Akt and MAPK protein expressions in cells were significantly reduced under the influence of different concentrations of melezitose.Conclusion Melezitose may exert anti-liver fibrosis effects by acting on multiple targets such as PI3K/Akt and MAPK.
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