Effect of emodin on neuroinflammation in bacterial meningitis rats by regulating cGAS/STING signaling pathway
Objective To investigate emodin affect the neuroinflammation of bacterial meningitis rats by regulating cGAS/STING signaling pathway.Methods According to random number table method,60 rats were divided into control group,model group,emodin group,penicillin group,emodin+SR-717 group,with 12 rats in each group.The bacterial meningitis rat model was established by injecting B group hemolytic streptococcus(GBS)bacteria solution into the brain with stereotactic apparatus in 4 groups except control group.After the model was successfully made,rats in emodin group were ip with 20 mg/kg emodin,rats in penicillin group were ip with 10 mL/kg penicillin,rats in emodin+SR-717 group were ip with 20 mg/kg emodin and 30 mg/kg SR-717.The rats in control and model group were injected with the same amount of normal saline.The neurological symptoms score amd cerebrospinal fluid white blood cell(WBC)count were performed 3 d after administration.HE staining was applied to detect pathological changes in brain tissue,ELISA was applied to detect the content of IL-6 and TNF-α in brain tissue;the apoptosis of rat cortical neurons was detected by TUNEL staining.Western blotting was applied to detect the expression of cGAS and STING relative protein expression in brain tissue.Results Compared with the model group,the neurological symptom score,WBC count in cerebrospinal fluid,content of IL-6 and TNF-α in brain tissue,number of TUNEL stained nerve cells,protein expression of cGAS and STING relative protein expression in the emodin group were significantly decreased(P<0.05),brain tissue damage wes obviously reduced.Compared with emodin group,the neurological symptom score,WBC count in cerebrospinal fluid,content of IL-6 and TNF-α in brain tissue,number of TUNEL stained nerve cells,protein expression of cGAS and STING relative protein expression in emodin+SR-717 group were significantly increased(P<0.05),brain tissue damage worsened again.Conclusion Emodin may reduce neuroinflammation in bacterial meningitis rats by inhibiting the cGAS/STING signaling pathway.
emodincyclic guanosine monophosphate-adenosine monophosphate synthasestimulator of interferon genebacterial meningitisneuroinflammation
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