Effects of baicalein on inhibition of deep vein thrombosis in rats
Objective To evaluate the inhibition effect of baicalein on deep vein thrombosis in rats,and explore the mechanism.Methods 72 Male SD rats were randomly divided into the control group,model group,warfarin(0.27 mg/kg)group,and baicalein group(5,10,20 mg/kg).Except for the control group,each group was administered by gavage once daily for 4 days.The rat complete stasis model of inferior vena cava thrombosis was used,and the weight of thrombus formed in the inferior vena cava of each group was determined.The morphology and venous wall thickness of the inferior vena cava were observed by HE stained pathological sections.The plasma levels of P-selectin and IL-6,and the content of platelets GPIIb/IIIa in rats were determined by ELISA.Meanwhile,the rat tail bleeding time and clotting time were measured.Results Compared with the model group,baicalein 10 and 20 mg/kg group reduced the weight of thrombus formed in inferior vena cava,the thickness of the inferior vena cava vessel wall,and the P-selectin level(P<0.05,0.01).Compared with the model group,baicalein 20 mg/kg group reduced the IL-6 level and the content of glycoprotein IIbIIIa on platelet(P<0.01).Compared with the model group,baicalein 5,10,and 20 mg/kg group prolonged the bleeding time of rat tail(P<0.05,0.001).Conclusion Baicalein has the activity of inhibiting venous thrombosis in vivo,and its mechanism may be related to its reduction of inflammatory factors,and affecting the function of platelets.
baicaleindeep vein thrombosisthrombus weightP-selectinIL-6bleeding time
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