Application effectiveness of next-generation sequencing technology for pathogenic microorganisms in bronchoalveolar lavage fluid on refractory pneumonia
Objective To explore the application value of next-generation sequencing (NGS) technology for pathogenic micro-organisms in bronchoalveolar lavage fluid in the treatment of refractory pneumonia,providing a reference for clinical treat-ment.Methods A total of 200 patients with refractory pneumonia admitted to the Second People's Hospital of Hengshui from June 2021 to June 2023 were selected.Patients were randomly allocated to a control group and an observation group,with 100 cases in each group.The control group received empirical treatment after traditional detection methods for bron-choalveolar lavage fluid,while the observation group received precise treatment after NGS detection.The positive rate of pathogens,treatment outcomes,and hospitalization indicators were compared between the two groups.Inflammatory factor levels before and after treatment were also compared.Results The overall positive rate of pathogens in the observation group was significantly higher than in the control group (P<0.05).Before treatment,no significant difference was ob-served in white blood cell count,CRP,SAA,and PCT levels between the two groups (P>0.05).After treatment,these levels were significantly lower in both groups,with the observation group showing lower levels than the control group (P<0.05).The CURB 65 score,APACHE Ⅱ score,and MSCT abnormal imaging changes were significantly lower in the ob-servation group compared to the control group (P<0.05).The cure rate in the observation group was significantly higher than in the control group (P<0.05).The observation group also had a significantly shorter duration of mechanical ventila-tion,shorter length of hospital stay,and lower hospitalization costs compared to the control group (P<0.05).Conclusion Precise treatment following NGS detection of bronchoalveolar lavage fluid is more effective for patients with refractory pneu-monia,as it increases the detection rate of pathogenic bacteria,reduces inflammatory factors,alleviates the severity of pneu-monia,raises cure rates,and reduces medical costs,offering marked economic and social benefits.