地质聚合物管状无机膜固定化碳酸酐酶用于CO2捕获
Geopolymer-based inorganic membrane loaded with carbonic anhydrase for CO2 capture
赵宝中 1贺艳 1邓兴发 1肖培 1崔学民1
作者信息
- 1. 广西大学化学与化工学院,广西南宁 530004;广西石化资源加工及过程强化技术重点实验室,广西南宁 530004
- 折叠
摘要
为了研究多孔材料对酶固定化性能的影响,提出了一种以地质聚合物管状无机膜为载体的新型酶固定化策略.利用该膜材料介孔中存在的大量硅羟基和铝羟基结构,采用戊二醛交联法成功地将碳酸酐酶负载到该无机介孔膜中.固定化实验结果表明:固定化酶复合膜的酶活性回收率可达63.7％.在稳定性测试中,该膜在连续使用30次后保持了 67.8％的相对活性,在4 ℃下保存60 d后保持了 52.0％的相对活性.在CO2捕集试验中,CO2捕集效率在固定化酶复合膜催化下提高了 5.6倍.
Abstract
To study the effect of porous materials on the performance of enzyme immobilization,we proposed a novel strategy for enzyme immobilization using a geopolymer tubular inorganic membrane(GM)as a carrier.Carbonic anhydrase(CA)was successfully loaded into this mesoporous membrane using glutaraldehyde(GA)cross-linking,relying on the large number of silica hydroxyl and aluminum hydroxyl structures naturally present in the membrane's mesopores.The results of the immobilization experiments show that the immobilized enzyme composite membrane(GM-CA)achieves 63.7％enzyme recovery.In stability tests,the membrane retained 67.8％relative activity after 30 consecutive uses and 52.0％relative activity after 60 days of storage at 4 ℃.In the CO2 capture assay,the CO2 capture efficiency developed 5.6 times under the catalytic of GM-CA.
关键词
地聚物/碳酸酐酶/无机膜/二氧化碳捕获/二氧化碳分离Key words
geopolymer/carbonic anhydrase/inorganic membrane/CO2 capture/CO2 sepa-ration引用本文复制引用
基金项目
广西自然科学基金(2022GXNSFDA035062)
出版年
2024
NETL
NSTL
万方数据