首页|黄浆水与木薯粉混合发酵高浓度乙醇的发酵条件

黄浆水与木薯粉混合发酵高浓度乙醇的发酵条件

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  • 国家科技期刊平台
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以黄浆水与木薯粉混合同步糖化发酵高浓度乙醇,应用Plackett-Burman设计对底物浓度、尿素、糖化酶、液化酶、MgSO4、pH值、CaCl2、KH2PO48个因素进行2水平12次试验,研究发酵的最佳条件.结果发现,底物浓度、尿素、糖化酶和KH2PO4的添加量是影响发酵的重要因素,最佳发酵条件是底物浓度38％、尿素0.15％、糖化酶1.45AGU/g、KH2 PO40.07％.在最佳发酵条件下,乙醇浓度达到17.21％ (V/V).
Techniques for Fermentating High-concentration Ethanol from Mixing Yellow Serofluid and Cassava
The conditions of simultaneous saccharification and fermentation by mixing yellow se-rofluid and cassava flour were investigated for producing high-concentration ethanol. The important parameters were screened by Plackett-Burman design, and subsequently the path of steepest ascent was used to approach to the biggest region of ethanol production. Finally, the optimum values of parameters were obtained by orthogonal tests. The experimental results showed the optimum technical conditions as follows:Substrate concentration 38%,CO(NH2)2 0. 15%,Dextrozyme 1.45 AGU/g substrate,KHzPO40. 07%,under which the ethanol reached 17. 21% (V/V) after 48 h fermentation at 32°C and 100 r/min.

yellow serofluid, cassava flour, simultaneous saccharification and fermentation,PLackett-Burman design,ethanol

秦艳、申乃坤、莫勇生、朱婧、王青艳、廖思明

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广西科学院国家非粮生物质能源工程技术研究中心,广西南宁530007

广西科学院生物研究所,广西南宁530007

广西红树林研究中心,广西北海536000

黄浆水 木薯粉 同步糖化发酵 Plackett-Burman设计 乙醇

广西科学研究与技术开发计划项目广西区科学研究与技术开发课题广西区科学研究与技术开发课题广西科学院基金

桂科重12118004-3桂科攻1099070桂科攻11107008-410YJ25SW07

2012

广西科学
广西科学院 广西壮族自治区科学技术协会

广西科学

CSTPCD
影响因子：0.516
ISSN：1005-9164
年,卷(期)：2012.19(3)
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