Very High Gravity Fuel Ethanol Production with the Fermentation of Uncooked Cassava
[Objective]Very high gravity fuel ethanol production was studied with the fermentation of uncooked cassava flour.[Metheds]First,the important parameters were screened by single factor tests.Second,the optimum values of the parameters were obtained by response surface methodology.And finally,a gradient temperature control strategy was used with the optimized conditions,all of which were used to improve the ethanol concentration.[Results]The conditions optimized by single factor tests were starch hydrolyzing enzyme dosage 0.8 GAU/g cassava flour,substrate concentration 36 ％ (W/V),and initial pH value 4.2.The optimal concentrations were determined by response surface methodology as follows:Starch hydrolyzing enzyme dosage 0.82 GAU/g cassava flour,substrate concentration 37％,and initial pH value 4.3.A gradient temperature control strategy could reduce the reducing sugar and imporve the fermentation efficience.Under such optimum conditions,the ethanol concentration could reach up to 16.24％ (V/V),while the reducing sugar and total sugar were 0.29 ％ (W/V) and 1.81 ％ (W/V) correspondingly,after 96 h fermentation.The ethanol content of fermentation mashing increased 25 ％ compared with the original condition.[Conclusion]On the basis of integration technology,very high gravity fuel ethanol production and low production cost with the fermentation of uncooked cassava flour may facilitate industrial scale application in future.
fermentation of uncooked materialsresponse surface methodologyfuel ethanolcassavatemperature staging strategy
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