Revealing the level of genetic diversity and genetic differentiation of the rare and endangered plant Cycas sexseminifera population,and determining the priority populations for protection are of great signifi-cance for the effective protection and management strategy of C.sexseminifera.In this experiment,Simple Sequence Repeats(SSR)primers were designed based on simplified genome sequence analysis,and SSR prim-ers were used to detect genetic diversity.A total of 6 pairs of SSR primers were obtained in the experiment,and a total of 37 observed alleles(Na)were detected by 6 pairs of primers.The minimum number of ob-served alleles was 3,the maximum number of observed alleles was 12,and the average number of alleles per locus was 6.167.GZST002,GZST055,GZST065 and GZST088 were highly polymorphic loci(PIC＞0.5).The expected heterozygosity(He)of each population of C.sexseminifera was 0.330-0.533,with an aver-age value of 0.444,indicating that the genetic diversity of C.sexseminifera was at a moderate level.The He values of 7 populations were in the order of Guangxi Botanical Research Institute(ZWS)＞Chongzuo Guang-he(GH)＞Chongzuo Pairu(PR)＞Long'an Longhuai(LH)＞Chongzuo Zhonggan(ZG)＞Baise Zuodeng(ZD)＞Long'an Xinhui(XH).The fixation coefficient(F)ranged from-0.161 to 0.480,with an average value of 0.074.Among them,the three wild populations of LH,PR and XH were negative,and the fixed coef-ficients of the three wild populations of GH,ZD and ZG were greater than 0.The order of F value of 7 popu-lations was Baise Zuodeng(ZD)＞Guangxi Botanical Research Institute(ZWS)＞Chongzuo Zhonggan(ZG)＞Chongzuo Guanghe(GH)＞Long'an Longhuai(LH)＞Long'an Xinhui(XH)＞Chongzuo Pairu(PR).Therefore,based on various genetic indicators,the wild population of C.sexseminifera that needs to be protected and introduced is the Chongzuo Guanghe(GH)population,which has relatively high genetic diver-sity.At the same time,it is suggested to develop more SSR primers and collect a wider range of wild popula-tions of C.sexseminifera,and accurately screen excellent populations with high genetic diversity,so as to fa-cilitate a more comprehensive and accurate assessment of the genetic diversity level and endangered mecha-nism of C.sexseminifera.
维普
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