Prostate Cancer(PCa)is a malignant disease with great harm to middle-aged and elderly men.In recent years,the incidence and mortality of prostate cancer in China have increased significantly.In the past,systematic 6-core biopsy was the most widely used method for the diagnosis of prostate cancer in clinical practice,but this method could not significantly improve the detection rate of prostate cancer.With the pro-gress of imaging technologies such as single photon Computed Tomography(ECT),Multi-slice spiral CT(MDCT),Transrectal of Ultrasound(TRUS)and multi-parameter Magnetic Resonance(mpMRI),as well as the application of various fusion technologies in practical operation,prostate puncture has become accurate and visible,the positive rate of pathological diagnosis has been improved,and the number of puncture needles and complications have been reduced.This article reviews the new progress of prostate biopsy in recent years.