Effects of Multi-layer Mixed-age Modification on Soil Phosphor-us Components and Transformation in Cunninghamia lanceola-ta Plantation
The purpose of this study was to investigate the effects of multi-layer mixed-age introduction of broad-leaved tree species into Cunninghamia lanceolata plantation on soil Phosphorus(P)components and transformation.The soil physicochemical properties and P components were measured in the unmodified Pure C.lanceolata Plantation(PP)and the Mixed C.lanceolata Plantation(MP)after multi-layer mixed-age modification.The soil microbial community composition and enzyme activity were determined by high-throughput sequencing and 96-well plate,respectively.Furthermore,the relationship of soil physicochemical properties and P components with soil microbial community and enzyme activity was clarified.The results showed that:(l)Compared with those in PP,the Resin Phosphorus(RP),Labile Phosphorus(LP),Medium Availability Phosphorus(MAP)and Oculude Phosphorus(OP)in MP were significantly increased by 9.43％,9.20％,11.64％and 11.71％,respectively,and the Phosphorus Activation Coefficient(PAC)was significantly increased by 27.13％.(2)The Microbial Biomass Carbon(MBC),Microbial Biomass Nitrogen(MBN)and Microbial Biomass Phosphorus(MBP)in MP significantly increased by 13.52％,31.11％and 55.78％,respectively,compared with those in PP.The number of nodes,the number of edges and the average weighting degree of the microbial co-occurrence network in MP were higher than those in PP.(3)Compared with PP,MP increased the activities of β-Glucosidase(BG)and Cellobiohydrolase(CB)related to Carbon(C)metabolism by 175.58％(P＜0.01)and 21.81％(P＜0.05),the activity of N-Acetyl-Glucosylase(NAG)re-lated to Nitrogen(N)metabolism by 113.30％(P＜0.001),and the activity of Acid Phosphatase(ACP)in-volved in P metabolism by 71.49％(P＜0.01).There were significant positive correlations between the activ-ities of enzymes associated with C,N and P metabolism.(4)Soil Organic Carbon(SOC),Total Nitrogen(TN)and Fine Root Biomass(FR)were the main factors driving the changes of soil P components.In con-clusion,multi-layer mixed-age modification promotes soil P transformation and improves P availability in C.lanceolata plantation by altering the plant and soil physicochemical properties and increasing the content of P components and activities of enzymes involved in soil C,N and P metabolism.This approach may pave a new way for the alleviation of soil P limitation in subtropical C.lanceolata plantation.
万方数据
维普
