Effect of ubr1 Deletion on Mitotic Dynamics in Schizosaccharo-myces pombe
To explore the changes in mitotic dynamics after the deletion of ubr1 in Schizosaccharomyces pombe,this study employed fluorescent protein labeling and live cell imaging to compare the cell morphology and dynamics during mitosis between ubr1-deleted strain(ubr1Δ)and the Wild-Type(WT).The results showed that compared with WT,ubr1Δ showcased decreased cell aspect ratio,increased microtubule bundles,and appearance of short rod and pear-shaped cells.During mitosis,18.5％of ubr1Δ cells mistakenly formed double kinetochores,with the spindle elongation length increased by(1.58±0.76)μm and the spindle elon-gation duration prolonged by(3.00±1.36)min in anaphase compared with the WT.Meanwhile,the spindle exhibited fishhook and S-shaped breaks with delayed spindle breaks in telophase.In addition,compared with WT,ubr1Δ showed the increases of(1.12±0.19)μm in the initial diameter of the actin ring,(3.60±2.85)min in the actin ring formation time,and(4.90±0.21)min in the actin ring contraction time,and no signifi-cant difference in the contraction speed.The findings provide a scientific basis for further research on the function and molecular mechanism of ubr1 in mitosis.
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