Screening and Denitrification Performance of a Highly Ammonia Nitrogen-Tolerant Strain
Biological nitrogen removal by nitrification and denitrification is considered as one of the effective technologies of ammonia nitrogen-containing wastewater treatment,the key of which is the screening of effi-cient denitrifying microorganisms.In this study,a heterotrophic nitrifying-aerobic denitrifying bacterial strain,named Acinetobacter junii YQ-1,was isolated from a swine wastewater treatment pond,and the growth performance and denitrification capability of strain YQ-1 were investigated.The results showed that the highest cell density(OD595=0.999)was achieved at 30 ℃ after 48 h of cultivation with a rotation speed of 200 r/min,initial pH value 8,and an inoculum amount of 2.0％(V/V).Under the optimal growth condi-tions,strain YQ-1 showed the nitrogen removal rates of 89.73％,63.51％,and 70.19％within 48 h against ammonia nitrogen,nitrite nitrogen,and nitrate nitrogen at the initial concentration of 200 mg/L,respectively.Strain YQ-1 exhibited good growth even when the ammonia nitrogen concentration was 1 000 mg/L,with the growth fitting the BidoseResp model.The changes in Chemical Oxygen Demand(COD)in the culture medi-um followed the ExpDec2 model,and the rate of decrease in COD was positively correlated with the growth rate of YQ-1.This study provides a reference for the application of heterotrophic nitrifying-aerobic denitrif-ying bacteria in processing the wastewater containing high-concentration ammonia nitrogen.
万方数据
维普
