The edible safety of the water extract from Malus doumeri(Bois.)A.Chev.leaves was examined by acute oral toxicity and genotoxicity tests.According to the experimental method of National Food Safety Standard-Procedure for Toxicological Evaluation of Food Safety,the toxicity of M.doumeri(Bois.)A.Chev.leaves was evaluated by acute toxicity and genotoxicity tests(including Ames test,mammalian erythro-cyte micronucleus test,and chromosome aberration test of mouse spermatogonia).The results showed that the median lethal dose(LD50)of the water extract from M.doumeri(Bois.)A.Chev.leaves was greater than 20 000 mg/kg BW.In the range of 8-5 000 μg/dish,the water extract showed a negative result in the Ames test.Within the dose range of 2 500-10 000 mg/kg BW,the water extract did not cause damage to mouse bone marrow erythrocytes or lead to chromosome aberration of mouse spermatogonia.According to the re-sults,the water extract of M.doumeri(Bois.)A.Chev.leaves was non-toxic and demonstrated no genotoxic-ity under the test conditions.
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