Identification,Biological Characterization and Fungicide Screen-ing for Anthracnose Pathogen in the Seedling Stage of Smilax glabra
In order to identify and characterize the pathogen of anthracnose in the seedling stage of Smilax glabra,and screen out effective fungicides for the prevention and control of this disease.The pathogen of an-thracnose was isolated from S.glabra,and the pathogenicity of the isolate was determined.Morphological characteristics and multiple gene sequences(ITS,TUB,ACT,GAPDH and CHS)were used to identify the pathogen.Furthermore,the biological characteristics of the pathogen were examined,and the indoor toxicity test was carried out for four fungicides against this pathogen.The results showed that Colletotrichum theo-bromicola was the pathogen causing anthracnose in the seedlings of S.glabra.The optimal culture conditions for the mycelial growth of the pathogen were Sabourand's medium,full light,28 ℃,and pH value of 4.The optimal carbon sources were maltose and glucose,and the optimal nitrogen sources were potassium nitrate and peptone.The lethal temperature of mycelium was 55 ℃.The four fungicides showed strong toxicity and inhibitory effects on the mycelial growth of the pathogen.Among them,450 g/L prochloraz Water Dispersible Granule(WG)was the most toxic,with the median Effect Concentration(EC50)of 0.030 8 mg/L.The tox-icity of 250 g/L propiconazole Emulsifiable Concentrate(EC)was the weakest,with the EC50 of 0.561 7 mg/L.The results provide a scientific basis for the identification and prevention of anthracnose in the cultivation of S.glabra.
万方数据
维普
