Response Surface Methodology-Based Extraction Process Optimi-zation and Antioxidant Activity of Total Flavonoids from Hors-fieldia hainanensis
A response surface methodology was adopted to optimize the extraction process of total flavonoids from Horsfieldia hainanensis.Furthermore,the content and antioxidant activity of total flavonoids extracted by the optimal process at different growth stages(adult trees,young trees,and seedlings)and from different plant parts were measured.The results were yielded as follows:① The optimal extraction process for total flavonoids from H.hainanensis was extraction at the solid-liquid ratio of 1∶72.2 and 55.1℃ for 55.7 min.②The overall content of total flavonoids was higher in adult trees,with the highest content in the bark,reac-hing 16.061％.Seedlings had the second overall content of total flavonoids,which was the highest(10.465％)in leaves.The overall content of total flavonoids was the lowest in young trees,in which the branch bark had the highest content of 7.413％.③The trunk bark and seed coat of mature trees exhibited the strongest 1,l-diphenyl-2-picrylhydrazyl radical(DPPH •)scavenging capacity.The total flavonoids extrac-ted from leaves of mature trees showed strong hydroxyl radical(• OH)scavenging capability.The trunk of mature trees exhibited the highest superoxide radical(O2-•)scavenging rate.In conclusion,H.hainanensis has high content of total flavonoids,which demonstrate remarkable antioxidant activity,indicating the great medicinal value of this plant.The results of this study aim to provide a scientific basis for the rational devel-opment and application of H.hainanensis resources.
万方数据
维普
