Genetic Diversity of Acanthus ebracteatus Based on Microsatel-lite Markers
Nine polymorphic microsatellite loci were selected to study the genetic diversity of 4 populations of Acanthus ebracteatus,and the endangerment causes of A.ebracteatuswere identified.The results showed that 61 alleles were detected at 9 polymorphic microsatellite loci in 4 populations,with an average of 6.778 alleles per locus and the Polymorphism Information Content(PIC)of 0.589.The mean number of alleles per popu-lation was 3.167,and the mean observed Heterozygosity(Ho)and expected Heterozygosity(He)were 0.396 and 0.442,respectively.The analysis of molecular variance(AMOVA)showed that 36％and 64％of genetic variation were among and within populations,respectively,which indicated that the genetic variation mainly came from different individuals within populations.The results of cluster analysis and Principal Co-ordinates Analysis(PCoA)were consistent.The four populations were classified into two groups.The popu-lation from Guangzhou,Guangdong was clustered into one group,while those from Zhanjiang,Guangdong,Fangchenggang,Guangxi and Wenchang,Hainan were clustered into the other group.The four populations had rich genetic diversity,and the clustering results were consistent with the geographical distribution of each population.The endangerment of A.ebracteatusmay be caused by human activities,breeding,diseases and in-sect pests rather than the low genetic diversity.This study provides a theoretical basis for the conservation,development and utilization of A.ebracteatus.
万方数据
维普
