Comparison the Content of Hyperoside of Malus Doumeri（Bois） Chev. from Different Producing Area
The content of hyperoside of Malus doumeri（Bois） Chev.from different producing area was determined by HPLC.The C_18 ODS was used at the temperature of 30℃.The mobile phase consisted of acetonitrile and 0.1% orthophosphoric acid（12∶88） with isocratic elution.The UV detector was set at 360nm with 1.0ml/min flow rate.Results showed that a new standard curve,y=1832.3x＋15.938 and r=0.9999,was established.When the hyperoside sample size was between 0.032～1.28μg,the standard curve showed a good linear relationship with 98.9% average recovery and 2.22% RSD.According to the results from HPLC,the content of hyperoside of Malus doumeri（Bois） Chev.was various from different producing area.The highest content of hyperoside was found in Malus doumeri（Bois） Chev.produced in Hezhou.
国家科技期刊平台
NSTL
万方数据
维普
