【目的】通过多种现代化波谱手段，探究海洋细菌Bacillus sp。753的次生代谢产物。【方法】采用硅胶柱层析、Sephadex LH-20凝胶柱层析和高效液相色谱分离技术，对海洋细菌Bacillus sp。753发酵液的化学成分进行研究，并运用 N MR、MS和文献对照方法鉴定其化学结构。【结果】从这株细菌的发酵液中共分离得到12个单体化合物，分别为 uridine (1)，thymine (2)，cyclo-(phe-lle)(3)，cyclo-(ala-pro)(4)，cyclo-(val-pro)(5)， cyclo-(pro-leu)(6)，cyclo-(phe-pro)(7)，cyclo-(tyr-pro)(8)，cyclo-(tyr-4-hydroxy-pro)(9)，cyclo-(leu-pro)(10)，cyclo-(pro-4-hydroxyl-lle)(11)，cyclo-(D-pipecolinyl-L-leucine)(12)。【结论】化合物1~12均是首次从该株海洋细菌中分离得到，该实验结果不仅丰富了该株菌的化学成分类型，而且也增强了海洋细菌的研究价值。
Analysis of the Secondary Metabolites of Marine Bacte-rium Bacillus sp.753
[Objective]To investigate the secondary metabolites of marine bacterium Bacillus sp.753 by various modern spectrum methods.[Methods]Constituents in the fermentation liq-uid of the marine bacteria were isolated and purified by silica gel,Sephadex LH-20,and semi-preparative reverse-phase HPLC (C18 ).Their structures were identified by NMR,MS and lit-eratures.[Results]Twelve compounds were iso-lated from marine bacterium Bacillus sp.753 and their structures were identified as uridine (1 ), thymine (2),cyclo-(phe-lle)(3),cyclo-(ala-pro) (4),cyclo-(val-pro)(5),cyclo-(pro-leu)(6),cy-clo-(phe-pro)(7),cyclo-(tyr-pro)(8),cyclo-(tyr-4-hydroxy-pro)(9),cyclo-(leu-pro)(10), cyclo-(pro-4-hydroxyl-lle)(11),cyclo-(D-pipecolinyl-L-leucine)(12).[Conclusion]Com-pounds 1~1 2 were isolated from this bacterium for the first time,which not only enriched the types of chemical composition in the Bacillus sp.753,but also increased the research val-ue of the marine bacterium.
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