In order to investigate the contents of total flavonoids,and total polysaccharides and antioxidant ac-tivities in three species of Cycas plants,C.ferruginea,C.segmentifida and C.sexseminifera,the contents of total flavonoids and total polysaccharides from their roots,petioles,leaves,male flowers and stems were extracted by ultrasonic-assisted method,and their antioxidant activities were evaluated by DPPH free radical(DPPH·),hydroxyl radical(-OH)and superoxide anion radical(O2-·)scavenging rates.Pearson correla-tion analysis was used to analyze correlation.The results showed that the contents of total flavonoids in the leaves of C.ferruginea,C.segmentifida and C.sexseminifera were relatively higher,and the order was C.ferruginea(8.61 mg·100 mg-1)>C.segmentifida(7.82 mg·100 mg-1)>C.sexseminifera(1.04 mg· 100 mg-1).The content of total polysaccharides in the stems was the highest,and the order was C.segmen-ti fida(28.32 mg·100 mg-1)>C.sexseminifera(24.43 mg·100 mg-1)>C.ferruginea(16.59 mg·100 mg-1).The scavenging ability of DPPH·was better in the leaves of C.ferruginea and C.segmentifida.The scavenging ability of·OH was poor in all parts of the three species of Cycas plants,and the scavenging ability of O2-·was better in the roots of C.segmentifida.In summary,the antioxidant activity of each part of the three Cycas plants is mainly related to the content of flavonoids.This study can provide a scientific ba-sis for the comprehensive utilization of Cycas plants in Guangxi.