Structure Characteristics of the Water Microbial Community during Seedling Stage of Paphia textile Larvae
In order to explore the composition and structure characteristics of water microbial community in Paphia textile larvae at different developmental stages,and to provide a theoretical basis for large-scale arti-ficial breeding of seedlings,high-throughput sequencing technology was used to sequence and analyze the wa-ter microbial community of larvae at D-shaped larval stage(HD),shell top stage(HK),pre-metamorphosis stage(HB),post metamorphosis stage(HA)and juvenile shell stage(HZ).The results of Alpha diversity index showed that the abundance and diversity of microbial communities in HD were the highest,while the abundance of microbial communities in HZ was the lowest and the diversity of microbial communities in HB was the lowest.At the phylum level,the dominant bacterial communities in all stages of larval development were Proteobacteria[including Alphaproteobacteria and Gammaproteobacteria],Bacteroidota and Actinobac-teriota.At the family level,the Rhodobacteriaceae and Flavobacteriaceae were the dominant groups in the whole breeding cycle.The relative abundance of Microbacteriaceae was less than 1%in HB,and gradually in-creased in HA and HZ,which were 9.95%and 12.82%,respectively.The relative abundance of Cryomor-phaceae was less than 1%in HD,and the relative abundance in HK,HA and HZ was higher,which was 5.98%-7.67%.It is worth noting that the relative abundance of Idiomarinceae had significant differences at different stages of P.textile larval development.The relative abundance of Idiomarinceae in HD and HK was less than 1%,but increased rapidly to 31.90%in HB.In summary,the diversity and composition of water mi-crobial community in different developmental stages of P.textile larvae changed significantly.