Research on the Allelopathy of Akashiwo sanguinea to Phaeo-cystis globosa
Both Akashiwo sanguinea and Phaeocystis globosa are the algae that frequently cause red tides in China's coastal waters in recent years.In order to explore whether there is allelopathy between A.sanguinea and P.globosa and how does it work,in this study,A.sanguinea and P.globosa were co-cultured at different initial densities,and the complete cell culture medium,cell-free filtrate and ultrasonic fragmentation medium of algae liquid of the former were mixed with P.globosa respectively to analyze the effect of A.sanguinea on the growth of P.globosa cells.The results showed that the growth of P.globosa with initial cell densities of 5 × 105,1 × 106,2 × 106,and 1 × 107cells/L was significantly inhibited when the initial cell density of A.san-guinea was maintained at 5 ×105 cells/L.The growth of P.globosa was significantly inhibited by either the culture medium containing intact cells of A.sanguinea,the cell-free filtrate or the ultrasonic fragmentation medium of algae liquid(P＜0.01).The order of inhibition intensity from strong to weak was complete cell culture medium,cell-free filtrate and ultrasonic fragmentation medium of algae liquid.On the contrary,the presence of P.globosa had no significant effect on the cell growth of A.sanguinea(P＞0.05).Therefore,A.sanguinea has the allelopathic effect of inhibiting the growth of P.globosa,and its allelochemicals are mainly secreted outside the cells.
red tideAkashiwo sanguineaPhaeocystis globoseallelopathyco-culture
万方数据
维普
