Research Progress in the Synthesis of Low Molecular Weights Dextran by Single-Enzyme Method
Low molecular weight dextran is synthesized by acid hydrolysis/enzymatic hydrolysis of glucose polymers catalyzed by Dextransucrase(DSR)using sucrose as a substrate.The molecular weight is 10 000-20 000 Da,which is widely used in medicine,food and other industries.In this article,the research progress on single enzyme synthesis of dextran were reviewed,including the advantages and disadvantages of microbial/enzymatic production of dextran,the efficient expression of DSR,the process regulation of single enzyme syn-thesis of dextran,the immobilization technology,the structure and catalytic mechanism of DSR,as well as the current research status of single enzyme synthesis of low molecular weight dextran.Based on this,the idea of DSR single enzyme one-step synthesis of low molecular weight glucan is proposed.With the help of artificial intelligence technology,more detailed DSR structure information is obtained.On the basis of further analy-zing the structure,function and catalytic mechanism of DSR,it is designed and improved reasonably,and the enzymatic properties are optimized to realize the direct synthesis of low molecular weight dextran by single enzyme one-step method,which provides new theoretical support for the production of low molecular weight dextran.
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